In the realm of immune monitoring, the process of cell proliferation stands as a crucial indicator of immune system activity and health. Cell proliferation refers to the rapid increase in cell numbers through division, a process tightly regulated within the immune system to maintain equilibrium and respond effectively to pathogens or abnormal cells. [1]
Cell proliferation serves as a fundamental mechanism underlying immune response dynamics. When the immune system encounters foreign invaders such as viruses, bacteria, or cancer cells, it initiates a cascade of events aimed at eliminating the threat. Central to this response is the rapid expansion of specific immune cell populations, including T cells, B cells, and natural killer cells. Monitoring the proliferation of these cells provides invaluable insights into the strength and effectiveness of the immune response. [2]
At PeploBio, we utilise cutting-edge technology to assess cell proliferation, including the BD FACSLyric clinical cell analyser, an IVD-grade instrument renowned for its precision and reliability. This state-of-the-art analyser enables us to perform comprehensive analyses of immune cell dynamics with unparalleled accuracy and efficiency.
At PeploBio, we employ a multifaceted approach to assess cell proliferation, encompassing various assays and techniques.
A programmed cell death process crucial for maintaining immune homeostasis. Apoptosis is evaluated through parameters such as phosphatidylserine exposure, assessed using Annexin V binding assays, mitochondrial changes, caspase activation, and DNA fragmentation detected through assays like BrdU incorporation. [3]
A fundamental aspect of cell proliferation regulation. By analysing cellular DNA content using techniques such as propidium iodide (PI) versus 7-aminoactinomycin D (7-AAD), we can determine cell cycle phases and assess S phase activity through BrdU incorporation.
Cyclin-dependent kinases, and cell cycle regulators such as cyclin B1, providing a holistic understanding of cell cycle progression and regulation. [4]
Flow cytometry emerges as a powerful tool for studying cell proliferation within the immune system. This sophisticated technique enables the simultaneous analysis of multiple cellular characteristics, including cell surface markers, intracellular proteins, and DNA content. By labelling cells with fluorescent markers and passing them through a flow cytometer, researchers can quantify proliferation rates, identify distinct cell populations, and track changes in immune cell dynamics over time. [5]
