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Flow Cytometry Services: Precision Cell Analysis for Research & Diagnostics
Flow cytometry is a powerful analytical technique used in various fields of research, diagnostics, and beyond. Discover the limitless potential of flow cytometry at PeploBio. Our flow cytometry services are tailored to meet your unique needs, whether you're in research, diagnostics, or pharmaceuticals. Backed by a team of experts and industry-standard technology, we provide the accuracy and quality your projects demand.
At PeploBio, we understand that every research project is unique, and precision is key. That's why we offer a comprehensive suite of Flow Cytometry services, meticulously designed to cater to your specific needs. Our experienced team combines cutting-edge technology with scientific expertise to provide you with accurate and actionable insights.
Immunophenotyping Services
Immunophenotyping is a crucial technique in understanding immune cell populations and surface markers. With the help of fluorescent antibodies, we can accurately characterise and analyze different immune cell subsets, helping you gain deeper insights into the intricacies of the immune system. Whether you're exploring immune responses, investigating diseases, or developing targeted therapies, our immunophenotyping services provide the precision you need.
Receptor Occupancy
Flow cytometry-based Receptor Occupancy (RO) assays are rapid, powerful, and customisable tools in precision medicine. RO assays are designed to quantify and characterize the binding capabilities of therapeutic drugs to their target receptors on the cell surface [46, 47]. RO assays can also be used to assess other bioactive substances such as neurotransmitters and hormones but spotlight in clinical platforms tend to fall on therapeutic drugs due to their vast applications in medicine.
Cell Cycle Analysis
Understanding the cell cycle is fundamental in many areas of research, from cancer biology to developmental biology. Our cell cycle analysis service provides valuable insights into the progression of cells through their life cycle. By studying DNA content and cell cycle phases, you can uncover critical information about cell division, proliferation, and even identify abnormalities. This knowledge is invaluable for a wide range of applications, including drug development and disease research.
Apoptosis Detection Services
Programmed cell death, or apoptosis, plays a crucial role in tissue homeostasis and disease processes. Our apoptosis detection service allows you to investigate this phenomenon with exceptional sensitivity. By employing cutting-edge techniques, we can identify apoptotic cells and quantify their levels accurately. Whether you're studying apoptosis as part of cancer research or drug development, our services provide the precision required for meaningful results.
DNA Content Analysis
The integrity of DNA is central to genetic research and diagnostics. With our DNA content analysis service, you can assess the DNA content of individual cells with remarkable accuracy. Whether you're analyzing DNA ploidy, genome stability, or studying chromosomal abnormalities, our technology and expertise are at your disposal. Trust in PeploBio to provide the insights you need for your genetic investigations.
Specialised Services Using Flow Cytometry
We understand that research projects often come with unique challenges that demand custom solutions. That's where our specialized services come into play. Whether it's developing novel assays, optimising protocols, or addressing unconventional research requirements, our team is ready to collaborate with you. We pride ourselves on our ability to find creative and effective solutions, ensuring that your research objectives are met with precision and excellence.
Need more info?
Dive deeper into our services by contacting us today. Our experts are ready to provide you with all the information you need.
The process begins with the preparation of the sample. Cells or particles of interest are suspended in a fluid and may be labeled with fluorescent markers (fluorochromes) or antibodies that bind to specific molecules on their surface.
Fluidics System
The sample is injected into a flow cytometer's fluidics system, which creates a single-cell suspension and controls the flow rate to ensure that cells or particles pass through the laser beam one at a time.
Laser Excitation
As each cell or particle passes through the flow cell, it encounters a focused laser beam. The laser excites the fluorochromes or dyes attached to the particles, causing them to emit fluorescence.
Light Scattering
Simultaneously, the laser light scatters off the cell or particle. There are two types of light scattering measurements: Forward Scatter (FSC) and Side Scatter (SSC).
Fluorescence Detection
The emitted fluorescence is collected by detectors at various wavelengths. Each detector corresponds to a specific fluorochrome or emission wavelength. This allows the flow cytometer to capture multiple parameters simultaneously.
Data Analysis
The data collected from each cell or particle is processed and analyzed by a computer. The results are typically presented in the form of histograms or dot plots.
More about Flow Cytometry
Frequently Asked Question
What is flow cytometry, and how does it work?
Flow cytometry, a pivotal technique in bioanalytical testing and precision medicine, involves passing cells or particles through a fluid stream. Using a laser beam and fluorescent antibodies, it measures light scattered and emitted by the samples to provide insights into their characteristics.
What are the applications of flow cytometry?
Flow cytometry finds applications in diverse fields, including immune cell analysis, cell cycle studies, and generating flow cytometry data. Researchers rely on it for its versatility and utility in various scientific investigations.
What types of samples can be analyzed with flow cytometry?
Flow cytometry is suitable for analyzing a wide range of sample types, such as blood, tissue, cultured cells, and microorganisms. Its flexibility and fluorescent markers make it adaptable to different research needs.
What are the available markers and fluorochromes for our specific research needs?
We provide a selection of fluorescent dyes and multicolor flow cytometry options to cater to your specific research requirements. These tools enable you to identify target molecules or characteristics in your samples accurately.
What is the cost of flow cytometry services?
The cost of our flow cytometry services varies depending on your needs, including cell size, fluidics system, and dot plots. Contact us for a personalized quote, considering your research goals.
What is the turnaround time for flow cytometry analysis or sorting?
The turnaround time for flow cytometry analysis or sorting depends on factors like the complexity of your project and whether you require single cell suspension or single file analysis. We strive to provide efficient results and can discuss specific timelines with you.
Do you provide data analysis and interpretation services?
Yes, we offer data analysis and interpretation services to assist you in deriving valuable insights from your flow cytometry analysis. Our experts can help you understand and interpret the light scattered and fluorescence activated cell sorting data effectively.
Can you help with experimental design and panel optimization?
Certainly, our team can assist in designing experiments and optimizing panels using our flow cytometer. We'll guide you in selecting appropriate markers and antibodies for your research, ensuring successful experiments.
Is cell sorting available, and what are the options for sorting cells based on specific criteria?
We provide cell sorting services with options to sort cells based on specific criteria using fluorescence activated cell sorting technology. Our capabilities can be tailored to meet your precise research needs.
What are the instrumentation and technology platforms available for flow cytometry?
We employ advanced instrumentation and technology platforms in our flow cytometry services. Our flow cytometers utilize state-of-the-art technology to ensure precise and reliable results.
Is there a minimum or maximum sample size requirement?
Sample size requirements can vary depending on the analysis or sorting method you choose. Feel free to contact us to discuss your specific sample quantity requirements.
What are the storage and handling requirements for samples before analysis?
Proper sample preparation and storage are essential for accurate results. We can provide guidelines on how to prepare and store your samples, including recommendations for cell cycle studies and more.
Are there any sample preparation services offered, such as cell staining or fixation?
Yes, we offer sample preparation services, including cell staining and fixation, to ensure your samples are prepared optimally for analysis or sorting.
What quality control measures are in place to ensure data accuracy and reliability?
To maintain data accuracy and reliability, we implement rigorous quality control measures throughout the flow cytometry process, adhering to international harmonisation standards.
Can I schedule a facility tour or consultation to discuss my project in detail?
Absolutely, you can schedule a facility tour or consultation with our team to discuss your project in depth. We'll provide insights into our flow cytometric capabilities and address your specific research objectives.
Are there any restrictions or guidelines for hazardous or infectious samples?
If you are working with potentially hazardous materials, we have safety protocols and guidelines in place to ensure the safety of our staff and the environment. Technical harmonisation is a priority in handling such samples.
Do you offer training or educational resources on flow cytometry techniques?
Yes, we provide training and educational resources for users, including those new to flow cytometry. Our goal is to support researchers in gaining expertise in this valuable technique while adhering to international harmonisation standards.
Contact us to find out more
We are here to answer your questions, discuss potential collaborations, and explore how our our solutions can benefit you.
